Product Name: EIF2S1 Antibody
Species Reactivity: Dog, Human, Mouse, Rat, Zebrafish
Tested Applications: ELISA, IHC, WB
Applications: EIF2S1 antibody can be used for detection of EIF2S1 by ELISA at 1:2500. EIF2S1 antibody can be used for detection of EIF2S1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 35 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S1.
Host Species: Rabbit
Purification: Antibody is purified by protein A chromatography method.
Physical State: Lyophilized
CAS NO.: 500287-72-9
Product: Rilpivirine
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF2S1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Accession NO.: NP_004085
Protein Ino: 4758256
Official Symbol: EIF2S1
Geneid: 1965
Background: The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD), and gamma (52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha.The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987).
PubMed ID:http://aac.asm.org/content/39/7/1609.abstract
