Product Name: EGF Receptor / EGFR Antibody [GFR/1708]
Species Reactivity: Human
Tested Applications: Flow, IF, IHC-P, WB
Applications: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTFlow Cytometry: 0.5-1 ug/million cells in 0.1mlOptimal dilution of the EGF Receptor antibody should be determined by the researcher.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Purified recombinant human EGFR was used as the immunogen for the EGF Receptor antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 1138549-36-6
Product: CX-5461
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Accession NO.:
Protein Ino:
Official Symbol: EGFR
Geneid: 1956
Background: Epidermal growth factor receptor (EGFR) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha. Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of several tyrosine (Y) residues in the C-terminal domain of EGFR occurs. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding SH2 domains. These downstream signaling proteins initiate several signal transduction cascades, principally the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. [Wiki]
PubMed ID:http://aac.asm.org/content/39/6/1259.abstract
