Product Name: EGF Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect hEGF by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hEGF. Sandwich: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human EGF (XP-5119) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEGF.Western Blot:To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF (human Epidermal Growth Factor).
Host Species: Rabbit
Purification: Anti-hEGF specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 75507-68-5
Product: Flupirtine (Maleate)
Buffer:
Concentration:
Storage Conditions: EGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: URG, HOMG4Pro-epidermal growth factor, Urogastrone, EGF
Accession NO.: P01133
Protein Ino: 251757262
Official Symbol: EGF
Geneid: 1950
Background:
PubMed ID:http://aac.asm.org/content/39/6/1243.abstract
