Product Name: EGF Antibody
Species Reactivity: Human
Tested Applications: ELISA, Neut, WB
Applications: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL), a concentration of 0.25 0.35 μg/mL of this antibody is required. ELISA:To detect hEGF by direct ELISA (using 100us antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions. Sandwich: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEGF. Western Blot:To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight: 6.2 kDa
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF.
Host Species: Rabbit
Purification: Anti-hEGF specific antibody was purified by affinity chromatography employing immobilized hEGF matrix.
Physical State: Lyophilized
CAS NO.: 94055-76-2
Product: Suplatast (Tosilate)
Buffer:
Concentration:
Storage Conditions: EGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: URG, HOMG4Pro-epidermal growth factor, Urogastrone, EGF
Accession NO.: P01133
Protein Ino: 251757262
Official Symbol: EGF
Geneid: 1950
Background:
PubMed ID:http://aac.asm.org/content/39/6/1240.abstract
