Product Name: E2F4 Antibody
Species Reactivity: Dog, Human, Mouse
Tested Applications: ELISA, WB
Applications: E2F4 antibody can be used for detection of E2F4 by ELISA at 1:312500. E2F4 antibody can be used for detection of E2F4 by western blot at 2.0/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 44 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F4.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 1026785-59-0
Product: VX-222
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store E2F4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: E2F4, E2F-4
Accession NO.: NP_001941
Protein Ino: 12669915
Official Symbol: E2F4
Geneid: 1874
Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
PubMed ID:http://aac.asm.org/content/39/3/714.abstract
