Product Name: DNTT Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: DNTT antibody can be used for detection of DNTT by ELISA at 1:62500. DNTT antibody can be used for detection of DNTT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 58 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DNTT.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 1018069-81-2
Product: TG 100801 (Hydrochloride)
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store DNTT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DNTT, TDT
Accession NO.: NP_004079
Protein Ino: 63054850
Official Symbol: DNTT
Geneid: 1791
Background: DNTT is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3-hydroxyl terminus of oligonucleotide primers. In vivo, DNTT is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
PubMed ID:http://aac.asm.org/content/38/11/2530.abstract
