Product Name: DDX60 Antibody
Species Reactivity: Mouse, Rat
Tested Applications: IF, WB
Applications: DDX60 antibody can be used for detection of DDX60 by Western blot at 1 – 2 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 125, 185, 188 kDa Observed: 190 kDa
Immunogen: DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.The immunogen is located within amino acids 1140 – 1190 of DDX60.
Host Species: Rabbit
Purification: DDX60 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 121584-18-7
Product: Valspodar
Buffer: DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: DDX60 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DDX60 Antibody: Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60
Accession NO.: NP_060101
Protein Ino: 222831595
Official Symbol: DDX60
Geneid: 55601
Background: DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.
PubMed ID:http://aac.asm.org/content/38/5/1085.abstract
