Product Name: DARPP 32 (phospho Thr34) Antibody
Species Reactivity: Human, Mouse, Rat, Xenopus
Tested Applications: WB
Applications: Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr34) recognizes human, mouse, and rat forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 32
Immunogen: DARPP-32 (Thr34) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr34 of DARPP-32.
Host Species: Rabbit
Purification: Affinity Purified
Physical State: Liquid
CAS NO.: 104632-28-2
Product: Dexpramipexole
Buffer: 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Concentration:
Storage Conditions: For long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚C.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Darpp32, Darpp-32, DARPP-32,
Accession NO.: Q6J4I0
Protein Ino: 81884861
Official Symbol: Ppp1r1b
Geneid: 360616
Background: DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
PubMed ID:http://aac.asm.org/content/38/2/378.abstract
