Product Name: DARC Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IHC-P, WB
Applications: DARC antibody can be used for detection of DARC by Western blot at 0.5 – 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: DARC antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human DARC.The immunogen is located within the last 50 amino acids of DARC.
Host Species: Rabbit
Purification: DARC Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1373615-35-0
Product: PF-5274857
Buffer: DARC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: DARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DARC Antibody: FY, Dfy, GPD, GpFy, ACKR1, CCBP1, CD234, WBCQ1, FY, Atypical chemokine receptor 1
Accession NO.: Q16570
Protein Ino: 67476970
Official Symbol: DARC
Geneid: 2532
Background: DARC Antibody: DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.
PubMed ID:http://aac.asm.org/content/38/2/374.abstract
