Product Name: DAP3 Antibody
Species Reactivity: Human
Tested Applications: ELISA, ICC, WB
Applications: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kDa is observed. This band is not observed in the non-transfe Immunocytochemistry: Mitochondrial staining of HeLa after in situ denaturation of the target protein using 3-4M guanidinium thiocyanate (patent pending). The subcellular localisation is in agreement with Morgan et al., Biochem Biophys Res
User Note:
Predicted Molecular Weight:
Immunogen: DAP3 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of DAP3.
Host Species: Goat
Purification: DAP3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 1396772-26-1
Product: EPZ005687
Buffer: DAP3 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Accession NO.: NP_387506.1, NP_004623.1
Protein Ino: 16905526
Official Symbol: DAP3
Geneid: 7818
Background:
PubMed ID:http://aac.asm.org/content/38/2/360.abstract
