Product Name: Cytokeratin Antibody [7H8C4]
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: Western Blot:1:500 – 1:2,000 IHC(P):1:500 – 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified truncated recombinant CK expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 686344-29-6
Product: Otenabant
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration:
Storage Conditions: Cytokeratin(Pan) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: 58 kDa cytokeratin, CK-5, K5, CK5, DDD, DDD1, EBS2, KRT5A
Accession NO.: P13647
Protein Ino: 143811411
Official Symbol: KRT5
Geneid: 3852
Background: Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
PubMed ID:http://aac.asm.org/content/38/1/122.abstract
