Product Name: Cyclin B1 Antibody [V92.1]
Species Reactivity: Hamster, Human, Mouse
Tested Applications: Flow, IF, IP
Applications: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlIP: 1-2ug/500ug protein–precipitates active Cyclin B1 complexesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Hamster protein was used as the immunogen for this Cyclin B1 antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 500-38-9
Product: Nordihydroguaiaretic acid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Accession NO.:
Protein Ino:
Official Symbol: CCNB1
Geneid: 891
Background: This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.
PubMed ID:http://aac.asm.org/content/37/9/2020.abstract
