Product Name: Cyclin A2 Antibody [E67]
Species Reactivity: African green monkey, Bovine, Human, Mouse
Tested Applications: Flow, IF, WB
Applications: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Full length bovine protein was used as the immunogen for this CCNA2 antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 1435488-37-1
Product: XMD17-109
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Cyclin-A2, Cyclin-A, CCNA2, CCN1, CCNA
Accession NO.:
Protein Ino:
Official Symbol: CCNA2
Geneid: 890
Background: This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk’s. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.
PubMed ID:http://aac.asm.org/content/37/9/2000.abstract
