Product Name: Cullin 3 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC, IP, WB
Applications: Cullin 3 antibody can be used in ELISA starting at 1:1000, and immunohistochemistry starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Cullin 3 antibody was raised against amino acids 1-12 from the N-Terminus of Cullin 3 (Human).
Host Species: Rabbit
Purification: Delipidation and Defibrination
Physical State: Liquid
CAS NO.: 380315-80-0
Product: Tenovin-1
Buffer: 0.01% NaN3
Concentration:
Storage Conditions: Antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CUL3, Cullin 3, Cullin homolog 3, Cullin-3, KIAA0617, PHA2E, CUL-3
Accession NO.: Q13618
Protein Ino: 12643396
Official Symbol: CUL3
Geneid: 8452
Background: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1.
PubMed ID:http://aac.asm.org/content/37/8/1645.abstract
