Product Name: Collagen I Antibody
Species Reactivity: Mouse, Rat
Tested Applications: IHC, IP
Applications: Collagen I antibody can be used in ELISA, and immunohistochemistry starting at 1:50.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Collagen I antibody was raised against Collagen Type I from human and bovine placenta (Human).
Host Species: Rabbit
Purification: Immunoaffinity Chromatography
Physical State: Liquid
CAS NO.: 745-65-3
Product: Prostaglandin E1
Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Concentration:
Storage Conditions: Antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Alpha-1 type I collagenOI4
Accession NO.: P02452
Protein Ino: 296439504
Official Symbol: COL1A1
Geneid: 1277
Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce type specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
PubMed ID:http://aac.asm.org/content/36/11/2370.abstract
