Product Name: Collagen IV Antibody
Species Reactivity: Bovine, Human
Tested Applications: ELISA, IF, IHC, IP, WB
Applications: Collagen IV antibody can be used in immunohistochemistry starting at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Collagen IV antibody was raised against amino acids from the C-Terminus of Collagen Type IV from human and bovine placenta (Human).
Host Species: Rabbit
Purification: Immunoaffinity Chromatography
Physical State: Liquid
CAS NO.: 82834-16-0
Product: Perindopril
Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Concentration:
Storage Conditions: Store Collagen IV antibody at 4 ˚C prior to opening. This product is stable at 4 ˚C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20 ˚C or below.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ICH, HANAC, POREN1, arresten
Accession NO.: P02462
Protein Ino: 125987809
Official Symbol: COL4A1
Geneid: 1282
Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce type specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with type I, II, III, V, or VI collagens.
PubMed ID:http://aac.asm.org/content/36/11/2381.abstract
