Product Name: Collagen III Antibody
Species Reactivity: Bovine, Human
Tested Applications: ELISA, IHC, IP, WB
Applications: Collagen III antibody can be used in ELISA starting at 1:000 – 1:1000, and immunohistochemistry starting at 5 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Collagen III antibody was raised against Full length Collagen Type III with intact 3-dimensional helical structure from human and bovine placenta.
Host Species: Rabbit
Purification: Immunoaffinity Chromatography
Physical State: Liquid
CAS NO.: 107133-36-8
Product: Perindopril (erbumine)
Buffer: 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Concentration:
Storage Conditions: Store Collagen III antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: EDS4A
Accession NO.: P02461
Protein Ino: 124056490
Official Symbol: COL3A1
Geneid: 1281
Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted Glycine-X-Y triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce type specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
PubMed ID:http://aac.asm.org/content/36/11/2375.abstract
