Product Name: Caspase-8 Antibody [C15]
Species Reactivity: Human
Tested Applications: ICC, IP, WB
Applications:
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Recombinant human caspase-8 (aa 181-478).
Host Species: Mouse
Purification: >95% (SDS-PAGE)
Physical State: Liquid
CAS NO.: 179474-85-2
Product: Prucalopride (succinate)
Buffer: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Concentration: 0.5 mg/ml
Storage Conditions: Stable for at least 1 year after receipt when stored at -20˚C.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like Protease
Accession NO.: NP_001219
Protein Ino: 15718704
Official Symbol: CASP8
Geneid: 841
Background: Procaspase-8 belongs to the family of caspases. Binding of FasL to Fas leads to formation of a receptor complex at the cellular membrane, which was named DISC. The DISC consists of oligomerized receptors, the DD-containing adaptor molecule FADD, procaspase-8, procaspase-10 and c-FLIP. The DISC structure provides a platform for the oligomerization of procaspase-8 that allows two procaspase-8 homodimers to be in the close proximity leading to the initial activation of procaspase-8. At the first cleavage step, the N-terminal p43/p41 and the C-terminal p30 cleavage products are generated. Importantly, these cleavage products already possess catalytic activity. At the second cleavage step, p43/p41 and p30 are processed to p10 and p18, respectively, which leads to the generation of the active caspase-8 heterotetramer (p18/p10)2.
PubMed ID:http://aac.asm.org/content/43/5/1294.abstract
