Product Name: Caspase-12 Antibody (Small)
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IHC-P, WB
Applications: Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 46 kDa (uncleaved) Observed: 38 kDa
Immunogen: Caspase-12 antibody was raised against a synthetic peptide corresponding to 15 amino acids within the small cleavage product of murine caspase-12.The immunogen is located within the last 50 amino acids of Caspase-12 .
Host Species: Rabbit
Purification: Caspase-12 Antibody (Small) is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 894002-50-7
Product: LDN-212320
Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Small)
Concentration: 1 mg/mL
Storage Conditions: Caspase-12 antibody (Small) can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Caspase-12 Antibody (Small): Caspase-12, CASP-12
Accession NO.: CAA73532
Protein Ino: 2094806
Official Symbol: Casp12
Geneid: 12364
Background: Caspase-12 Antibody: (Small)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimers disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.
PubMed ID:http://aac.asm.org/content/43/5/1111.abstract
