Product Name: CRYBA1 Antibody
Species Reactivity: Human, Mouse
Tested Applications: IHC-P, WB
Applications: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 25 kDa
Immunogen: This CRYBA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human CRYBA1.
Host Species: Rabbit
Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
Physical State: Liquid
CAS NO.: 1062243-51-9
Product: Ro3280
Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration: 0.5 mg/ml
Storage Conditions: Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Beta-crystallin A3, Beta-crystallin A3, isoform A1, Delta4 form, Beta-crystallin A3, isoform A1, Delta7 form, Beta-crystallin A3, isoform A1, Delta8 form, CRYBA1, CRYB1
Accession NO.: P05813
Protein Ino: 2506317
Official Symbol: CRYBA1
Geneid: 1411
Background: Crystallins are separated into two classes:taxon-specific, or enzyme, and ubiquitous. The latter classconstitutes the major proteins of vertebrate eye lens and maintainsthe transparency and refractive index of the lens. Since lenscentral fiber cells lose their nuclei during development, thesecrystallins are made and then retained throughout life, making themextremely stable proteins. Mammalian lens crystallins are dividedinto alpha, beta, and gamma families; beta and gamma crystallinsare also considered as a superfamily. Alpha and beta families arefurther divided into acidic and basic groups. Seven protein regionsexist in crystallins: four homologous motifs, a connecting peptide,and N- and C-terminal extensions. Beta-crystallins, the mostheterogeneous, differ by the presence of the C-terminal extension(present in the basic group, none in the acidic group).Beta-crystallins form aggregates of different sizes and are able toself-associate to form dimers or to form heterodimers with otherbeta-crystallins. This gene, a beta acidic group member, encodestwo proteins (crystallin, beta A3 and crystallin, beta A1) from asingle mRNA, the latter protein is 17 aa shorter than crystallin,beta A3 and is generated by use of an alternate translationinitiation site. Deletion of exons 3 and 4 causes the autosomaldominant disease zonular cataract with sutural opacities.
PubMed ID:http://aac.asm.org/content/37/4/793.abstract
