Product Name: CRYAB Antibody
Species Reactivity: Human, Mouse
Tested Applications: ELISA, WB
Applications: CRYAB antibody can be used for detection of CRYAB by ELISA at 1:312500. CRYAB antibody can be used for detection of CRYAB by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 12 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRYAB.
Host Species: Rabbit
Purification: Antibody is purified by protein A chromatography method.
Physical State: Lyophilized
CAS NO.: 125314-64-9
Product: Ro 31-8220
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store CRYAB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CRYAB, CRYA2, CTPP2, HSPB5, MFM2, CMD1II, CTRCT16, HEL-S-101
Accession NO.: EAW67166
Protein Ino: 119587570
Official Symbol: CRYAB
Geneid: 1410
Background: Alpha crystallins are composed of: alpha-A and alpha-B, for acidic and basic, respectively. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
PubMed ID:http://aac.asm.org/content/37/4/769.abstract
