Product Name: CRTC1 Antibody
Species Reactivity: Bovine, Human, Mouse, Pufferfish, Rat, Zebrafish
Tested Applications: ELISA, IF, IHC, WB
Applications: CRTC1 antibody can be used in ELISA starting at 1:14000 – 1:80000, Western Blot starting at 1:100 – 1:8000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: CRTC1 antibody was raised against amino acids 19 – 34 of CRTC1 (Human).
Host Species: Rabbit
Purification: Immunoaffinity Chromatography
Physical State: Liquid
CAS NO.: 923032-38-6
Product: Refametinib (R enantiomer)
Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration:
Storage Conditions: Antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CRTC1, KIAA0616, MECT1, TORC-1, TORC1, Transducer of CREB protein 1, WAMTP1
Accession NO.: Q6UUV9
Protein Ino: 68565585
Official Symbol: CRTC1
Geneid: 23373
Background: MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1, Transducer of regulated cAMP response element-binding protein 1, TORC1, and Transducer of CREB protein 1) is a nuclear protein that functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11;19) (q21;p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein.
PubMed ID:http://aac.asm.org/content/37/4/722.abstract
