Product Name: CHFR Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: CHFR antibody can be used for detection of CHFR by ELISA at 1:312500. CHFR antibody can be used for detection of CHFR by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 69 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHFR.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 14484-47-0
Product: Deflazacort
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHFR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CHFR, FLJ10796, FLJ33629, RNF116, RNF196
Accession NO.: NP_060693
Protein Ino: 239048907
Official Symbol: CHFR
Geneid: 55743
Background: CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of Lys-63-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at Lys-63 are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
PubMed ID:http://aac.asm.org/content/36/1/50.abstract
