Product Name: CD32 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 34kDa band observed in Human myeloid erythroblast cell line K562 and Human monocytic cell line U937 lysates (calculated MW of 34.0kDa according to NP_003992.3;). In transfected HEK293 transiently expressing CD32 a band of approx. 40k Immunohistochemistry: In paraffin embedded Human Liver shows membranous staining in endothelial cells between the hepatocytes. Similar results were obtained in Human Placenta. Recommended concentration, 3-6μg/ml.
User Note:
Predicted Molecular Weight:
Immunogen: CD32 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of CD32.
Host Species: Goat
Purification: CD32 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 1610537-15-9
Product: VT-464
Buffer: CD32 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: FCGR2B, CD32, FCG2, FCGR2, IGFR2, Fc fragment of IgG, low affinity IIb, receptor for (CD32), Fc gamma RIIb, low affinity II
Accession NO.: NP_003992.3, NP_001002273.1, NP_001002274.1, NP_001002275.1,
Protein Ino: 50511930
Official Symbol: FCGR2B
Geneid: 2213
Background:
PubMed ID:http://aac.asm.org/content/34/7/1312.abstract
