Product Name: CARF Antibody
Species Reactivity: Dog, Human, Mouse, Rat
Tested Applications: ELISA, IHC, WB
Applications: CARF antibody can be used for detection of CARF by ELISA at 1:312500. CARF antibody can be used for detection of CARF by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 64 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CARF.
Host Species: Rabbit
Purification: Antibody is purified by protein A chromatography method.
Physical State: Lyophilized
CAS NO.: 326914-06-1
Product: MHY1485
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store CARF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CARF, CARF
Accession NO.: NP_060102
Protein Ino: 8923040
Official Symbol: CDKN2AIP
Geneid: 55602
Background: CARF was first cloned as a novel binding partner of ARF from a yeast-interactive screen. CARF and ARF colocalize in the perinucleolar region and have a collaborative function. In the nucleoplasm, CARF interacts with p53 and enhances its function. The p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2.
PubMed ID:http://aac.asm.org/content/43/4/944.abstract
