Product Name: CARD12 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: CARD12 antibody can be used for detection of CARD12 by ELISA at 1:62500. CARD12 antibody can be used for detection of CARD12 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 116 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CARD12.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 550368-41-7
Product: PNRI-299
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store CARD12 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CARD12, CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1
Accession NO.: NP_067032
Protein Ino: 40788015
Official Symbol: NLRC4
Geneid: 58484
Background: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.
PubMed ID:http://aac.asm.org/content/43/4/789.abstract
