Product Name: Aurora Kinase Antibody
Species Reactivity: Human
Tested Applications: ELISA, IP, WB
Applications: ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48kDa is observed. This band is not observe Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
User Note:
Predicted Molecular Weight:
Immunogen: Aurora Kinase antibody was raised against a 10 amino acid synthetic peptide near the C-Terminus of Aurora Kinase.
Host Species: Goat
Purification: Aurora Kinase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 117620-77-6
Product: 3-Cyano-7-ethoxycoumarin
Buffer: Aurora kinase antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, ser, AIRK1, AYK1, IAK1
Accession NO.: NP_003591.2
Protein Ino: 38327562
Official Symbol: AURKA
Geneid: 6790
Background:
PubMed ID:http://aac.asm.org/content/53/8/3511.abstract