Product Name: AURKB Antibody [13E8D3]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: Western Blot:1:500 – 1:2,000 ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified truncated recombinant AURKB expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 553-08-2
Product: Thonzonium (bromide)
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration:
Storage Conditions: AURKB monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora 1, AIM-1, AIK2, AurB, IPL1, PPP1R48, aurkb-sv1, aurkb-sv2
Accession NO.: Q96GD4
Protein Ino: 317373473
Official Symbol: AURKB
Geneid: 9212
Background: AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39 kDa),localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates Chromosomal segregation during mitosis and meiosis.It may regulates several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis.Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts arepresent at high levels in human thymus and fetal liver.ARK-2 protein levels are maximal during both S and G2/M phases
PubMed ID:http://aac.asm.org/content/53/8/3487.abstract
