Product Name: ATG4C Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: ATG4C antibody can be used for detection of ATG4C by Western blot at 1 – 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 50 kDa Observed: 57 kDa
Immunogen: ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C.The immunogen is located within the last 50 amino acids of ATG4C.
Host Species: Rabbit
Purification: ATG4C antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1303607-60-4
Product: SAR405838
Buffer: ATG4C antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: ATG4C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ATG4C Antibody: APG4C, AUTL1, AUTL3, APG4-C, APG4C, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase
Accession NO.: NP_835739
Protein Ino: 30410846
Official Symbol: ATG4C
Geneid: 84938
Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b; overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4).
PubMed ID:http://aac.asm.org/content/53/7/2998.abstract
