Product Name: ATF6 Antibody [AF6229]
Species Reactivity: Human
Tested Applications: WB
Applications: Western blot: 2-4 ug/ml (2)IHC (Paraffin): 5 ug/ml (1)ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. ATF6 is seen in western blotting between 75~90KD.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.
Host Species: Mouse
Purification: Protein G purified monoclonal antibody
Physical State: Liquid
CAS NO.: 1793053-37-8
Product: LLY-507
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.5 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha
Accession NO.:
Protein Ino:
Official Symbol: ATF6
Geneid: 22926
Background: ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.
PubMed ID:http://aac.asm.org/content/53/7/2887.abstract
