Product Name: Cdc2 (phospho Tyr15) Antibody
Species Reactivity: Dog, Human, Mouse, Rat, Xenopus
Tested Applications: WB
Applications: The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of SK-N-MC total cell extracts with phospho- and non-phosphopeptide competition were used to establish the phospho-specificity of the antibody. Immunolabeling of the Mr 34 kDa cdc2 protein is blocked by the Ser15 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot Blots (DB) and Western Blot (WB). Suitability for Immunohistochemistry (IHC) has not been determined. Drosophila, human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 34
Immunogen: Cdc2 (Tyr15) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Tyr15 of human, mouse, rat and xenopus cdc2.
Host Species: Rabbit
Purification: Affinity Purified
Physical State: Liquid
CAS NO.: 34381-68-5
Product: Acebutolol (hydrochloride)
Buffer: 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Concentration:
Storage Conditions: For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: LOC365842, CDC-like kinase 2, Protein Clk2
Accession NO.: Q5XI98
Protein Ino: 81910362
Official Symbol: Clk2
Geneid: 365842
Background: Cdc2 is a highly conserved protein serine kinase that plays a key role in regulation of the cell cycle. The ability of cdc2 to exercise control over the cell cycle is dependent upon the phosphorylation of Tyr15 in cdc2.
PubMed ID:http://aac.asm.org/content/35/7/1298.abstract
