Product Name: IL-8 Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:To detect hIL-8 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 – 0.4 ng/well of recombinant hIL-8. Sandwich:To detect hIL-8 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 – 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-8 (XP-5199Bt) as a detection antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-8. Western Blot:To detect hIL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-8 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-8 (human Interleukin-8).
Host Species: Rabbit
Purification: Anti-hIL-8 specific antibody was purified by affinity chromatography employing immobilized hIL-8 matrix.
Physical State: Lyophilized
CAS NO.: 84605-18-5
Product: FPS-ZM1
Buffer:
Concentration:
Storage Conditions: IL-8 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: NAF, GCP1, LECT, LUCT, NAP1, CXCL8, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, Interleukin-8, C-X-C motif chemokine 8, IL-8
Accession NO.: P10145
Protein Ino: 124359
Official Symbol: IL8
Geneid: 3576
Background: This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL-8 attracts neutrophils, basophils, and T-cells, but not monocytes.
PubMed ID:http://aac.asm.org/content/26/4/601.abstract
