Product Name: IL-6 Antibody (biotin)
Species Reactivity: Mouse
Tested Applications: ELISA, WB
Applications: ELISA:To detect mIL-6 by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-6. Sandwich:To detect mIL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-6 (XP-5194) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-6.Western Blot:To detect mIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-6 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-6 (murine IL-6).
Host Species: Rabbit
Purification: Anti-mIL-6 specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 1077-28-7
Product: Ro 46-2005
Buffer:
Concentration:
Storage Conditions: IL-6 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: Il-6, Il-6, Interleukin-6, B-cell hybridoma growth factor, IL-6
Accession NO.: P08505
Protein Ino: 124348
Official Symbol: Il6
Geneid: 16193
Background: IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL3 on multipotential hematopoietic progenitors.
PubMed ID:http://aac.asm.org/content/26/4/570.abstract
