Product Name: IL-6 Antibody [1.2-2B11-2G10]
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IL-6 when used with our biotinylated antigen affinity purified anti-human IL-6 (XP-5193Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.Western blot:To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.25-0.50 ng/lane, under reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: The monoclonal antibody was produced in BALB/c x C57BL/6 F1 mice using recombinant hIL-6 as the immunizing antigen.
Host Species: Mouse
Purification:
Physical State: Lyophilized
CAS NO.: 84104-71-2
Product: Kasugamycin (hydrochloride hydrate)
Buffer:
Concentration:
Storage Conditions: IL-6 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 – 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Interleukin 6, IFNB2, Interleukin-6, B-cell stimulatory factor 2, IL-6, HGF, HSF, BSF2
Accession NO.: P05231
Protein Ino: 124347
Official Symbol: IL6
Geneid: 3569
Background: IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. In addition, IL-6 appears to interact with IL2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 is a multifunctional 24 kDa protein originally discovered in the medium of RNA stimulated fibroblastoid cells. It is upregulated by IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, IL17 and downregulated by glucocorticoids IL4, TGF beta. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be as important as IL1 and TNF alpha in regulating the acute phase response. IL6 has also been implicated in regulating adipose mass.
PubMed ID:http://aac.asm.org/content/26/4/575.abstract
