Product Name: IL-5 Antibody (biotin)
Species Reactivity: Mouse
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect Murine IL-5 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-5. SandwichTo detect Murine IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine IL-5 (38-272) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-5.Western Blot:To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-5 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Murine IL-5 specific antibody was purified by affinity chromatography and then biotinylated.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 20633-67-4
Product: 9-Azido-Neu5DAz
Buffer:
Concentration:
Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: Il-5, Il-5, Interleukin-5, B-cell growth factor II, IL-5
Accession NO.: P04401
Protein Ino: 124342
Official Symbol: Il5
Geneid: 16191
Background:
PubMed ID:http://aac.asm.org/content/26/4/557.abstract
