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Product Name: IL-18 Binding Protein Antibody (biotin)
Species Reactivity: Mouse
Tested Applications: ELISA, WB
Applications: ELISA:To detect mIL-18 BP by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 – 0.30 μg/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIL-18 BP. SandwichTo detect mIL-18BP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IL-18BP (XP-5173) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-18BP.Western Blot:To detect mIL-18 BP by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-18 BP is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIL-18 BP (murine Interleukin-18 Binding Protein).
Host Species: Goat
Purification: Anti-mIL-18 BP specific antibody was purified by affinity chromatography and then biotinylated.
Physical State: Lyophilized
CAS NO.: 4696-76-8
Product: Aucubin
Buffer:
Concentration:
Storage Conditions: IL-18 Binding Protein antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: MC54L, Igifbp, IL-18BP, Interleukin-18-binding protein, Interferon gamma-inducing factor-binding protein
Accession NO.: Q9Z0M9
Protein Ino: 251757287
Official Symbol: Il18bp
Geneid: 16068
Background:
PubMed ID:http://aac.asm.org/content/26/2/228.abstract

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Author: Betaine hydrochloride