Product Name: IL-10 Antibody [15B9-1-2]
Species Reactivity: Mouse
Tested Applications: ELISA, Neut, WB
Applications: ELISA:Sandwich:In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 1600 pg/mL of recombinant murine IL-10 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-10 as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot:To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-10 (0.5 ng/mL), a concentration of 3.0-5.0 μg/mL of this antibody is required.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: E.coli derived Recombinant Murine IL-10
Host Species: Mouse
Purification: This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
Physical State: Lyophilized
CAS NO.: 82752-99-6
Product: Eupatilin
Buffer:
Concentration:
Storage Conditions: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: Interleukin 10Interleukin-10, Cytokine synthesis inhibitory factor, IL-10, CSIF, TGIF, GVHDS, IL10A
Accession NO.: NP_000563.1
Protein Ino: 10835141
Official Symbol: IL10
Geneid: 3586
Background:
PubMed ID:http://aac.asm.org/content/26/1/42.abstract
