Product Name: IEX-1 Antibody
Species Reactivity: Human, Mouse
Tested Applications: ELISA, IF, IHC-P, WB
Applications: IEX1 antibody can be used for detection of IEX1 by Western blot at 1 – 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 17 kDa Observed: 17 kDa
Immunogen: IEX1 antibody was raised against a 24 amino acid synthetic peptide near the center of human IEX1.The immunogen is located within amino acids 40 – 90 of IEX-1.
Host Species: Rabbit
Purification: IEX-1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1333146-24-9
Product: Sancycline
Buffer: IEX-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: IEX-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: IEX-1 Antibody: DIF2, IEX1, PRG1, DIF-2, GLY96, IEX-1, IEX-1L, DIF2, Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Protein DIF-2
Accession NO.: P46695
Protein Ino: 3123229
Official Symbol: IER3
Geneid: 8870
Background: IEX-1 Antibody: IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-α. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-κB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.
PubMed ID:http://aac.asm.org/content/25/2/157.abstract
