Product Name: GLS Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: IF, IHC-P, WB
Applications: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:25For IF starting dilution is: 1:25For IF starting dilution is: 1:25
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 73 kDa
Immunogen: This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
Host Species: Rabbit
Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
Physical State: Liquid
CAS NO.: 1000025-07-9
Mc-Val-Cit-PABC-PNP
Buffer: Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration: 0.5 mg/ml
Storage Conditions: Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
Accession NO.: O94925
Protein Ino:
Official Symbol: GLS
Geneid: 2744
Background: Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
PubMed ID:http://aac.asm.org/content/18/3/409.abstract
