Product Name: Filaggrin Antibody [FLG/1561]
Species Reactivity: Human
Tested Applications: Flow, IF, IHC, WB
Applications: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTOptimal dilution of the Filaggrin antibody should be determined by the researcher.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Amino acids 998-1104 of human FLG were used as the immunogen for this Filaggrin antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 1227158-85-1
BAY 87-2243
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: FLI1 Antibody : ATOD2, Filaggrin
Accession NO.:
Protein Ino:
Official Symbol: FLI1
Geneid: 2312
Background: Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
PubMed ID:http://aac.asm.org/content/41/2/471.abstract
