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Product Name: FasL Antibody [Mike-2]
Species Reactivity: Human
Tested Applications: Flow, WB
Applications: FasL antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: Cell extracts from FasL transfected cells (5×104) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-FasL Mike-2 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: FasL (monoclonal Mike-1) antibody was raised against a recombinant human soluble FasL from amino acids 103-281.
Host Species: Rat
Purification: FasL (monoclonal Mike-1) antibody was purified to ≥95% in SDS-PAGE from concentrated hybridoma culture supernatant.
Physical State: Liquid
CAS NO.: 50-76-0
Actinomycin D
Buffer: PBS containing 0.02% sodium azide
Concentration:
Storage Conditions: FasL antibody can be stored at 4˚C, stable for one year.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: APT1LG1, CD95L, FASL, TNFSF6, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL, CD178, ALPS1B, CD95-L, APT1LG1
Accession NO.: P48023
Protein Ino: 1345957
Official Symbol: FASLG
Geneid: 356
Background:
PubMed ID:http://aac.asm.org/content/40/9/2043.abstract

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Author: Betaine hydrochloride