Product Name: FES Antibody [5A11G5]
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: Western Blot:1:500 – 1:2,000 IHC(F):1:500 – 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified truncated recombinant FES-His expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 209984-57-6
LY-411575
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration:
Storage Conditions: FES monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: FPS, Proto-oncogene c-FesFPS
Accession NO.: P07332
Protein Ino: 115502390
Official Symbol: FES
Geneid: 2242
Background: FES (feline sarcoma oncogene) and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases.FES and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains. FES was originally identified as an oncogene from avian and feline retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Mutations may activate the FES kinase and thereby contribute to cancer. However, recent data strongly suggests that the c-FES protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer.
PubMed ID:http://aac.asm.org/content/40/12/2698.abstract
