Product Name: Eosinophil Peroxidase Antibody [AHE-1]
Species Reactivity: Human
Tested Applications: Flow, IF
Applications: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome were used as the immunogen for this Eosinophil peroxidase antibody.
Host Species: Mouse
Purification: Protein G purified monoclonal antibody
Physical State: Liquid
CAS NO.: 152121-30-7
Product: SB 202190
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: EPX, EPO, EPP, EPX-PEN
Accession NO.:
Protein Ino:
Official Symbol: EPX
Geneid: 8288
Background: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).
PubMed ID:http://aac.asm.org/content/39/11/2520.abstract
