Product Name: C-raf Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, WB
Applications: C-raf antibody can be used for detection of C-raf by Western blot at 0.5 – 1 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 72 kDa Observed: 72 kDa
Immunogen: C-raf antibody was raised against an 19 amino acid synthetic peptide near the amino terminus of human C-raf.The immunogen is located within amino acids 30 – 80 of C-raf.
Host Species: Chicken
Purification: C-raf Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 129-77-1
Product: Piperidolate (hydrochloride)
Buffer: C-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: C-raf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: C-raf Antibody: NS5, CRAF, Raf-1, c-Raf, RAF, Proto-oncogene c-RAF, cRaf
Accession NO.: NP_002871
Protein Ino: 4506401
Official Symbol: RAF1
Geneid: 5894
Background: C-raf Antibody: C-raf is the cellular homolog of viral raf gene (v-raf) and encodes a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases. Once activated, C-raf phosphorylates and activates the protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine specific protein kinases, ERK1 and ERK2. These activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
PubMed ID:http://aac.asm.org/content/42/7/1731.abstract
