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Product Name: CD40 Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: CD40 antibody can be used for detection of CD40 by ELISA at 1:62500. CD40 antibody can be used for detection of CD40 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 20 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CD40.
Host Species: Rabbit
Purification: Antibody is purified by peptide affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 157843-41-9
Product: CNP-AFU
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store CD40 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CD40, Bp50, CDW40, MGC9013, TNFRSF5, p50
Accession NO.: NP_690593
Protein Ino: 23312371
Official Symbol: CD40
Geneid: 958
Background: CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3).The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
PubMed ID:http://aac.asm.org/content/34/9/1787.abstract

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Author: Betaine hydrochloride

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Product Name: CD40, Antibody
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 48kDa band observed in lysates of cell line Daudi (calculated MW of 30.6kDa according to NP_001241.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-3μg/ml.
User Note:
Predicted Molecular Weight: Approx 48kDa
Immunogen: CD40, Antibody was raised against synthetic peptide PQEINFPDDLPGSN found at an internal region of CD40.
Host Species: Goat
Purification: CD40, Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 122341-56-4
Product: AMPPD
Buffer: CD40 is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CD40, CD40 molecule, TNF receptor superfamily member 5, Bp50, CDW40, MGC9013, TNFRSF5, p50, B cell surface antigen CD40, B cell-associated molecule, B-cell surface antigen CD40, CD40 antigen (TNF receptor superfamily member 5), CD40 type II isoform, CD40L
Accession NO.: NP_001241.1
Protein Ino: 23312371
Official Symbol: CD40
Geneid: 958
Background:
PubMed ID:http://aac.asm.org/content/34/9/1816.abstract

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Author: Betaine hydrochloride