Product Name: CARM1 (Ab 228) Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: IF, WB
Applications: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
User Note:
Predicted Molecular Weight: 63 kDa
Immunogen: CARM1 (Ab-228) antibody was raised against a peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1 .
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Physical State: Liquid
CAS NO.: 1191911-27-9
Product: CZC-54252
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: PRMT4, PRMT4
Accession NO.: NP_954592.1
Protein Ino: 40288288
Official Symbol: CARM1
Geneid: 10498
Background: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg-17 and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-κ-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at Arg-2142, which may loosen its interaction with NCOA2/GRIP1, and at Arg-580 and Arg-604 in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
PubMed ID:http://aac.asm.org/content/43/4/947.abstract
