Product Name: Acidic Cytokeratin Antibody [ACCK1-2]
Species Reactivity: Human
Tested Applications: Flow, IF, IHC, WB
Applications: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the acidic Cytokeratin antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: A human partial recombinant KRT77 protein was used as the immunogen for this acidic Cytokeratin antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 1456632-41-9
Product: SH5-07
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names:
Accession NO.:
Protein Ino:
Official Symbol:
Geneid: 374454
Background: This mAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
PubMed ID:http://aac.asm.org/content/52/1/374.abstract
