Product Name: ACTH Antibody [SPM501]
Species Reactivity: Human
Tested Applications: ELISA, Flow, IF, IHC
Applications: ELISA: order BSA free format for coatingFlow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 ug/mlIHC (FFPE):0.5-1 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone (CLIP sequence) were used as the immunogen for this ACTH antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 315-22-0
Product: Monocrotaline
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: POMC, proopiomelanocortin, Precursor of MSH, POC, ACTH, CLIP, adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin, LPH, MSH, NPP
Accession NO.:
Protein Ino:
Official Symbol: POMC
Geneid: 5443
Background: ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
PubMed ID:http://aac.asm.org/content/52/2/716.abstract
