ant distinction, in comparison with the handle (DMSO). the (p 0.01) and3.three. BD1 Accession Effects of TEB on LipidofUptakeRegulation Proteins in HepG2 Cellsin HepG2 Cells 3.three. on Lipid TEB on Lipid Uptake Regulation Proteins three.three. Effects of TEB Effects Uptake Regulation Proteins in HepG2 Cells Remedy of cells with TEB (20, with and80 for 11h) drastically improved the increased the Remedy of Treatment of cells 40, and 80 for h) significantly drastically cells with TEB (20, 40, TEB (20, 40, and 80 for 1 h) improved the translocation of PPAR from the cytosol for the cytosol to comparedwith the handle cells handle cells translocation of PPAR from thenucleus, compared with the handle cells translocation of PPAR in the cytosol to nucleus, the nucleus, compared with all the (p 0.05) (Figure 3A). Additional, cells Further,with TEB (20, with and80 , 12 h) showed 12 h) showed treated with treated 40, and 80 , 12 h) 80 , (p 3A). (Figure cells (p 0.05) (Figure 0.05) Further,3A). treated cells TEB (20, 40, TEB (20, 40, and showed greater proteinhigher protein levelsFATP2 than the control cells (p 0.05)cells (p 3B). TEB greater proteinlevels of CD36 and FATP2 than the control cells (p 0.05)(Figure 3B). TEB levels of CD36 and of CD36 and FATP2 than the manage (Figure 0.05) (Figure 3B). TEB also markedlyalso markedly gene levels of gene levels of CD36,FATP5,when compared with the increased the enhanced the CD36, FATP2, and FATP5, when compared with in comparison with the also markedly increased the gene levels of CD36, FATP2, and FATP2, and FATP5, the handle (p 0.05) (Figure 3C). (Figure 3C). manage (p 0.05) control (p 0.05) (Figure3C).Foods 2021, 10, 2242 Foods 2021, 10,7 of7 ofFigure 3. Expression of lipid uptake and lipid oxidation-associated moleculesmolecules in HepG2 cells when Figure three. Expression of lipid uptake and lipid oxidation-associated in HepG2 cells when treated with TEB.with Protein amount of nuclear PPAR PPAR (1:three,000 dilution) in HepG2(B) protein treated (A) TEB. (A) Protein level of nuclear (1:3,000 dilution) in HepG2 cells; cells; (B) protein and and (C) mRNA levelslevels of CD36 (1:three,000 dilution), FATP2 (1:three,000 dilution), and FATP5 (1:three,000 dilution) in (C) mRNA of CD36 (1:3,000 dilution), FATP2 (1:3,000 dilution), and FATP5 (1:3,000 dilution) in cells; (D) protein levels of PPAR (1:1000 dilution) in the nucleus and (E) mRNA degree of cells; (D) protein levels of PPAR (1:1000 dilution) inside the nucleus and (E) mRNA degree of CPT1 in CPT1 in cells. Cells were exposed to 20, 40, and 80 TEB for 1 or 12 h (n = 3 wells/group). JNK medchemexpress Lipofercells. Cells were exposed to 20, 40, and 80 TEB for 1 or 12 h (n = 3 wells/group). Lipofermata mata (20 ) was added to the cells 1 h ahead of the TEB remedy. Lamin B and GAPDH have been employed (20 ) was added gene, respectively. The information are represented as imply APDH (p made use of as as housekeeping protein and to the cells 1 h before the TEB remedy. Lamin B and SEM. had been 0.05), (p housekeeping protein andshow arespectively. The information are represented as imply SEM. (p 0.05), 0.01), and (p 0.001) gene, substantial difference, when compared with the manage (DMSO). (p 0.01),0.01) show a 0.001) show a significant difference, TEB-treated cells. control (DMSO). # (p 0.05) and ## (p and (p substantial difference, compared to the when compared with the # (p 0.05) and ## (p 0.01) show a considerable difference, in comparison with the TEB-treated cells.three.4. Effects of TEB on Lipid Uptake Regulation in HepG2 Cells three.4. Effects of TEB on Lipid Uptake Re
