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D 0.51 0.21 a 4.07 0.77 e1.11 0.28 b 0.74 0.16 c 1.70 0.15 a 1.59 0.46 b 0.27 0.03 b 1.39 0.53 b 0.59 0.24 e nd 7.40 0.93 cSY, CS and ZJ represent Larou in the farms of Shaoyang City, Changsha City and Zhangjiajie City, in Hunan BIX-01294 trihydrochloride Autophagy Province, respectively. YPT, YSH and TRS are from factories in Hunan Province. NDMA, NMEA, NDEA, NDPA, NDBA, NPIP, NPYR and NMOR indicate N-nitrosodimethylamine, N-nitrosomethylethylamine, N-nitrosodiethylamine, N-nitrosodi-n-propylamine, N-nitrosodi-n-butylamide, N-nitrosopiperidine, N-nitrosopyrrolidine and N-nitrosomorpholine, respectively. The symbols represent the implies regular deviation obtained from three independent experiments. Indicates with distinct letters in the similar row are significantly unique (p 0.05, n = 3/group). “nd” indicates “Not detected”.Table 3. Content material of polycyclic aromatic hydrocarbons ( /kg) in Larou of artisanal and industrial origin. Compounds BaA Ch BbF BkF BaP DbA BgP PAH4 PAH8 Artisanal Origin SY 0.50 0.11 de 9.14 0.70 c 0.42 0.12 d 0.32 0.02 b 0.59 0.06 d 1.02 0.07 c 0.55 0.04 c 10.64 c 12.53 d CS 1.17 0.24 c 20.62 0.97 a 0.44 0.01 d 0.28 0.01 c 0.25 0.02 e 2.22 0.04 b 0.34 0.01 e 22.48 a 25.31 a ZJ 0.71 0.13 d 17.19 1.07 b 0.68 0.03 c 0.51 0.03 a 0.78 0.07 c 2.74 0.08 a 0.59 0.01 b 19.35 b 23.18 b YPT 0.20 0.02 e 0.95 0.01 f 0.16 0.01 e 0.11 0.01 d 0.30 0.06 e 0.49 0.01 f 0.39 0.01 d 1.62 d 2.61 e Industrial Origin YSH two.73 0.39 a 4.95 0.39 e two.36 0.20 a 0.52 0.02 a 1.87 0.11 a 0.80 0.20 d 0.86 0.02 a 11.92 c 14.ten c TRS 1.97 0.26 b 6.33 0.20 d 1.23 0.03 b 0.49 0.01 a 1.11 0.07 b 0.68 0.14 de 0.38 0.01 d 10.64 c 12.18 dSY, CS and ZJ represent Larou in the farms of Shaoyang City, Changsha City and Zhangjiajie City, in Hunan Province, respectively. YPT, YSH and TRS are from factories in Hunan Province. BaA, Ch, BbF, BkF, BaP, DbA and BgP indicate benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene and benzo-(g,h,i)perylene, respectively. The symbols represent the implies regular deviation obtained from 3 independent experiments. Signifies with distinct letters within the same row are drastically distinct (p 0.05, n = 3/group).three.5. Correlation Analysis among Parameters The PCA analysis and correlations among pH, Aw, TBARS, residual nitrite, NAs and PAHs from all Larou samples are shown in Figure 1 and Tables 4 and five, respectively. The two principal components accounted for 85.1 in the total variation (49.5 and 35.six , respectively) within the PCA model. The N-Palmitoyl dopamine supplier initial principal component (PC1) was connected with pH, Aw, some nitrosamines (NDPA, NDEA and NDMA) and PAHs, except for DbA. PC2 was positively connected with residual nitrite and contents of PAH and NA, which have been clustered in the same group. Similarly, there was a powerful correlation involving the total NAs and residual nitrites in all Larou solutions ( = 0.805, p 0.01), among which a good coefficient index between residual nitrites along with the amount of NDPA ( = 0.626, p 0.05), NDBA ( = 0.913, p 0.05) and NPYR ( = 0.596, p 0.05) had been observed (Table four). The NMEA, NPYR and TBARS were adjacently clustered in a group of PCA plot, indicating a constructive partnership. In addition, considerable optimistic correlations have been also discovered in between TBARS values and NDBA ( = 0.970, p 0.01), NPIP (0.869, p 0.05) and NPYR ( = 0.590, p 0.05). It was discovered that pH was positively correlated with NDEA ( = 0.671, p 0.01) and NDPA ( = 0.594, p 0.05), but negatively correlated with NMEA ( = -0.850, p.

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